Thermo Fisher Scientific Gaussia luciferase Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Luciferase

Published species

Human, Luciferase

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Purified recombinant Gaussia luciferase expressed in E. coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539912

Product Specific Information

Western blot analysis of PA1-181 detects a ~20 kDa protein in the supernatant of Gaussia luciferase-secreting cells.

PA1-181 can be used for activity-independent verification of Gaussia luciferase expression.

Target Information

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. Gaussia luciferase can be measured individually or in multiplex assays, such as the Gaussia-Firefly Luciferase Dual Assay Kit (Product # 16181). The Gaussia luciferase has been codon optimized for enhanced performance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.