Thermo Fisher Scientific Gaussia luciferase Polyclonal Antibody
Gaussia luciferase를 특이적으로 인식하는 Rabbit Polyclonal Antibody. Western blot에서 1:1,000~1:10,000 희석으로 검증됨. 비활성 의존적 발현 확인 가능. 단백질 G 정제, 액상 형태, -20°C 보관.
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Applications
- Western Blot (WB): 1:1,000–1:10,000
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Luciferase |
| Published Species | Human, Luciferase |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Purified recombinant Gaussia luciferase expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2539912 |
Product Specific Information
Western blot analysis of PA1-181 detects a ~20 kDa protein in the supernatant of Gaussia luciferase-secreting cells.
PA1-181 can be used for activity-independent verification of Gaussia luciferase expression.
Target Information
Gaussia luciferase is a 20 kDa protein derived from the marine copepod Gaussia princeps.
This bioluminescent enzyme is highly secreted into cell culture media, enabling live cell monitoring of reporter activity.
Light output from the luciferase reaction correlates with the amount of Gaussia luciferase protein produced, allowing assessment of promoter activity.
Gaussia luciferase can be used individually or in multiplex assays such as the Gaussia-Firefly Luciferase Dual Assay Kit (Product #16181).
The Gaussia luciferase has been codon optimized for enhanced performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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