Thermo Fisher Scientific CD43 Monoclonal Antibody (DFT-1), PE

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Neat

-

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

DFT-1

Immunogen

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Conjugate

PE PE PE

View additional formats

• FITC
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Lyophilized

Concentration

Conc. Not Determined

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_928323

Product Specific Information

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD43 antibody, clone DFT-1 recognizes human CD43 also known as leukosialin or sialophorin.

Target Information

CD43 (leukosialin, sialophorin) is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumor suppressor p53 protein, but in case of p53 and ARF deficiency CD43 promotes tumor proliferation and viability. CD43 appears to be an important modulator of leukocyte functions. Diseases associated with CD43 dysfunction include Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.