Thermo Fisher Scientific CRY1 Monoclonal Antibody (OTI2C10), TrueMAB

Applications and Tested Dilution

• Western Blot (WB): 1:2,000

Species Reactivity

• Human
• Mouse
• Rat

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

Circadian rhythms in biochemical, physiological, and behavioral processes are controlled by an internal biological clock. Cryptochrome 1 (CRY1) and Cryptochrome 2 (CRY2) are DNA-binding flavoproteins homologous to blue-light receptors and photolyases.
In Drosophila, CRY acts as a light-dependent photoreceptor regulating the TIM protein. In mammals, CRY1 and CRY2 interact in a light-independent manner with CLOCK, BMAL1, PER1, PER2, and TIM to regulate circadian gene expression.
Mutations in Cry1 or Cry2 alter locomotor activity rhythms, demonstrating that both proteins are essential for proper circadian synchronization. Double-mutant mice lacking both proteins exhibit arrhythmicity, indicating a loss of internal circadian rhythm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.