Thermo Fisher Scientific DNMT3B Polyclonal Antibody
DNMT3B 단백질을 인식하는 Rabbit Polyclonal 항체로, Human 및 Rat 시료에 반응합니다. Western blot, IHC, ICC, IP 등 다양한 응용에 사용 가능하며, 항원 친화 크로마토그래피로 정제되었습니다. PBS/glycerol buffer에 보관되며 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| Immunoprecipitation (IP) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.29 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792689 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Pig (85%).
- Positive Control: HeLa, HeLa nuclear, GFP-tagged DNMT3B-transfected 293T.
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
CpG methylation is an epigenetic modification important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have shown that DNA methylation is essential for mammalian development.
This gene encodes a DNA methyltransferase involved in de novo methylation rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated.
Mutations in this gene cause immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Six alternatively spliced transcript variants have been described, though the full-length sequences of variants 4 and 5 have not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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