Thermo Fisher Scientific ZEB2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

20 µg/mL

View 2 publications 2 publications

ChIP assay (ChIP)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid peptide near the carboxy terminus of human ZEB2. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZEB2,uniProtId:O60315-1,ncbiNodeId:9606,antigenRange:1214,antigenLength:1214,antigenImageFileName:PA5-20980_ZEB2_O60315-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20980_ZEB2_O60315-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153527

Product Specific Information

Due to post-translational modifications, ZEB2 migrates at ~180kDa in SDS-PAGE. A suggested positive control is Jurkat cell lysate.

PA5-20980 can be used with blocking peptide PEP-1094.

Target Information

ZEB2, initially identified as Smad interacting-protein 1, is normally located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Like the homologous ZEB1, ZEB2 inhibits the transcription of the E-cadherin gene and induces epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing, in vitro. ZEB2 can also protect cells from DNA damage-induced apoptosis, suggesting that its expression may contribute to tumor progression. Recent evidence has shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, indicating that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Hirschsprung disease/Mowat-Wilson syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.