Thermo Fisher Scientific VSV-G Epitope Tag Polyclonal Antibody, DyLight 680
VSV-G 에피토프 태그를 인식하는 토끼 폴리클로날 항체로, DyLight 680 형광 표지. Western blot, ICC/IF, ELISA에 적합. 고순도 친화 크로마토그래피 정제. 단백질 발현 검증 및 태그 검출에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 501–511 (Y-T-D-I-E-M-N-R-L-G-K) of vesicular stomatitis virus glycoprotein (VSV-G) conjugated to KLH using maleimide. |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate, pH 7.2, with 0.15 M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This affinity purified antibody is directed against the VSV-G epitope tag and is useful in determining its presence in overexpressed proteins in various assays. The antibody recognizes the VSV-G epitope tag (Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Target Information
Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The glycoprotein (VSV G) contains a domain in its extracellular membrane-proximal stem that appears to be needed for efficient VSV budding.
Sequence: YTDIEMNRLGK
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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