Thermo Fisher Scientific 6X His Tag Polyclonal Antibody, DyLight 680

Applications and Tested Dilution

Product Specifications

Product Specific Information

• Store vial at 4°C prior to restoration.
• For extended storage, aliquot and freeze at -20°C or below.
• Avoid freeze/thaw cycles.
• Centrifuge if not clear after standing at room temperature.
• Stable for several weeks at 4°C as an undiluted liquid.
• Dilute only prior to immediate use.

This affinity purified antibody is directed against the 6X HIS epitope tag and is useful for determining its presence in overexpressed proteins in various assays. The antibody recognizes the 6X HIS epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.

Target Information

The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins by fusing a known epitope, enabling analysis even when specific antibodies are unavailable.
His-tagged proteins can be purified efficiently due to the strong chelation between histidine clusters and metal ions such as Ni²⁺ on Ni²⁺-NTA adsorbents.
His tag-specific antibodies facilitate detection or co-immunoprecipitation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지