Thermo Fisher Scientific CD3 Monoclonal Antibody (RIV9)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

1:500

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Published species

Pig

Host/Isotype

Mouse / IgG3

Class

Monoclonal

Type

Antibody

Clone

RIV9

Immunogen

CD3, Human white blood cell fraction if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD3,uniProtId:P07766-1,ncbiNodeId:9606,antigenRange:1-207,antigenLength:207,antigenImageFileName:MA1-7630_CD3_P07766-1_House_mouse.svg,antigenImageFileNamePDP:MA1-7630_CD3_P07766-1_House_mouse_PDP.jpeg,sortOrder:1},{targetFamily:CD3,uniProtId:P04234-1,ncbiNodeId:9606,antigenRange:1-171,antigenLength:171,antigenImageFileName:MA1-7630_CD3_P04234-1_House_mouse.svg,antigenImageFileNamePDP:MA1-7630_CD3_P04234-1_House_mouse_PDP.jpeg,sortOrder:2},{targetFamily:CD3,uniProtId:P09693-1,ncbiNodeId:9606,antigenRange:1-182,antigenLength:182,antigenImageFileName:MA1-7630_CD3_P09693-1_House_mouse.svg,antigenImageFileNamePDP:MA1-7630_CD3_P09693-1_House_mouse_PDP.jpeg,sortOrder:5},{targetFamily:CD3,uniProtId:P20963-1,ncbiNodeId:9606,antigenRange:1-164,antigenLength:164,antigenImageFileName:MA1-7630_CD3_P20963-1_House_mouse.svg,antigenImageFileNamePDP:MA1-7630_CD3_P20963-1_House_mouse_PDP.jpeg,sortOrder:6}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.01% sodium azide

Storage conditions

4°C short term, -80°C long term

RRID

AB_2073347

Product Specific Information

MA1-7630 detects CD3 from human, rat and mouse samples.

MA1-7630 has been successfully used in ELISA, flow cytometry, immunofluorescence, immunoprecipitation, and immunohistochemistry (frozen) procedures.

The MA1-7630 immunogen is a CD3 protein.

Store at 4ºC short term. For long term storage, store at -80ºC.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.