Thermo Fisher Scientific IL-11 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MPGPPPGPPR VSPDPRAELD STVLLTRSLL ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human IL-11. Anti-Human IL-11-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human IL-11 by sandwich ELISA (using 100 µL/well), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-11 (500-P01) as a capture antibody, allows the detection of at least 2000–4000 pg/mL of Recombinant Human IL-11.

Western Blot:
To detect Human IL-11 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible development reagents, the detection limit for Recombinant Human IL-11 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P01BT-1MG is provided as 2 × 500 µg.

Target Information

Interleukins (ILs) are a large group of cytokines produced mainly by leukocytes, though some are produced by phagocytes and auxiliary cells. ILs direct immune cells to divide and differentiate, acting through specific receptors on target cell groups.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.