Thermo Fisher Scientific Biotin (Vitamin B7 or Vitamin H) Monoclonal Antibody (BTN, 36)
Biotin(Vitamin B7/H) 검출용 Mouse monoclonal antibody (Clone BTN, 36). Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 비보존액 PBS 용액 형태로 제공되며 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
| Immunomicroscopy (IM) | Assay-dependent |
| In Situ Hybridization (ISH) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BTN, 36 |
| Immunogen | Biotinylated sheep immunoglobulin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Target Information
Biotin is a water-soluble B complex vitamin, generally classified as Vitamin B7.
It is required by all organisms and can only be synthesized by bacteria, yeasts, molds, algae, and some plant species.
Biotin is essential for incorporation of carbon dioxide into organic compounds and has a molecular weight of approximately 244 Da.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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