Thermo Fisher Scientific Beta-catenin Monoclonal Antibody (OTI3F9), TrueMAB
Beta-catenin 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, Flow Cytometry에 적합. 다양한 종에 반응하며, 세포 접착 및 Wnt 신호 연구에 활용 가능. 동결건조 형태로 제공되며 재구성 및 접합 실험에 용이.
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Thermo Fisher Scientific Beta-catenin Monoclonal Antibody (OTI3F9), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3F9 |
| Immunogen | Full length human recombinant CTNNB1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Beta-catenin is an adherens junction (AJ) protein involved in cell-cell adhesion and signaling pathways regulating cell growth and morphogenesis. It interacts with E-cadherin and alpha-catenin, linking adhesion complexes to the actin cytoskeleton. Beta-catenin also associates with adenomatous polyposis coli protein and microtubules, participating in Wnt/Wingless signaling.
Pathologically, beta-catenin is implicated in pilomatrixoma, medulloblastoma, colorectal, ovarian, and other solid tumors. In the nucleus, it co-activates Lef/Tcf transcription factors, promoting transcription of genes like cyclin D and c-myc involved in cell proliferation and tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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