Thermo Fisher Scientific EIF4E2 Monoclonal Antibody (OTI1F2), TrueMAB
EIF4E2 단백질을 인식하는 Mouse IgG1 단일클론 항체로, Western Blot, IHC, Flow Cytometry에 적합. 인간 시료 반응성, 무보존제 제형, 동결건조 상태로 제공. 연구용으로 번역 개시 억제 관련 연구에 활용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F2 |
| Immunogen | Full-length human recombinant protein of EIF4E2 produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
EIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during early translation initiation. Acts as a repressor of translation initiation and competes with EIF4E, preventing eIF4F complex assembly.
[UniProt]
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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