Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70), PE
HCN1 단백질을 검출하기 위한 PE 결합 단일클론 항체로, 인간·생쥐·랫드 시료에 반응합니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 565/576 nm 형광 특성을 가집니다. 단백질 G 정제, 보존제 무첨가, 4°C 보관 권장.
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Thermo Fisher Scientific HCN1 Monoclonal Antibody (S70), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S70 |
| Immunogen | Fusion protein amino acids 778–910 (C terminus) of rat HCN1 |
| Conjugate | PE |
| Excitation/Emission Max | 565/576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931856 |
Available Formats
- Unconjugated (MA5-27589)
- APC (MA5-45399)
- FITC (MA5-45400)
- PerCP (MA5-45401)
- Custom conjugation available upon request
Product Specific Information
1 µg/mL of MA5-45402 was sufficient for detection of HCN1 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Detects approximately 100 kDa. No cross-reactivity against HCN2.
Target Information
HCN1 belongs to the hyperpolarization-activated cation channel family (HCN), which contributes to spontaneous rhythmic activity in both heart and brain.
HCN1 and HCN2 are critical for motor learning and neuronal integration by cerebellar Purkinje cells, influencing autonomous neuronal activity and network oscillations.
HCN1 expression is enriched in cerebral cortex, hippocampus, cerebellum, and facial motor nucleus.
HCN2 is abundant in mamillary bodies, pontine nucleus, ventral cochlear nucleus, and nucleus of the trapezoid body.
Regional specificity of HCN channels contributes to differences in neuronal pacemaker activity across brain systems.
Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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