Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgM (Heavy chain) Secondary Antibody, AP
Goat 유래 F(ab')2 형태의 anti-Human IgM (Heavy chain) 2차 항체로, AP(Alkaline Phosphatase) 결합. Western blot, IHC, ELISA 등 다양한 응용 가능. 높은 특이성과 감도를 제공하며, 친화 크로마토그래피로 정제됨. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human Mu immunoglobulin |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535954 |
| Target | IgM (Heavy chain) |
| Antibody Form | F(ab')₂ Fragment |
Additional Formats
Product Specific Information
Each lot of antibody is validated for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are used for detection, sorting, or purification of target antibodies.
Secondary antibodies enhance assay versatility and sensitivity via multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification.
Typically, they are generated by immunizing host animals with pooled human immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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