Thermo Fisher Scientific SARS-CoV-2 Spike Protein S1 Recombinant Rabbit Monoclonal Antibody (HL263)
SARS-CoV-2 Spike 단백질 S1을 특이적으로 인식하는 Recombinant Rabbit Monoclonal 항체입니다. SARS-CoV 및 MERS-CoV와 교차 반응하지 않으며, WB, IHC, ICC/IF, ELISA에 사용 가능합니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| ELISA | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL263 |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890588 |
Product Specific Information
This antibody detects SARS-CoV-2 Spike protein but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on internal testing.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV (2019 Novel Coronavirus), causes illnesses ranging from mild cold-like symptoms to severe respiratory distress and death.
The SARS-CoV-2 Spike Protein consists of two domains, S1 and S2, and serves as the main surface antigen of the virus. The glycosylated Spike protein, along with the nucleocapsid protein, can be detected in infected cell culture supernatants using antisera from SARS patients.
The S1 domain contains a receptor-binding domain (RBD) that specifically binds to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Variations in Spike proteins across coronaviruses reflect their diversity and influence receptor interactions and fusion mechanisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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