Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) Recombinant Rabbit Monoclonal Antibody (HL257)
SARS-CoV-2 스파이크 단백질(RBD)을 인식하는 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공. Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. SARS-CoV 및 MERS-CoV와 교차 반응 없음. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:3,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
| ELISA | Assay-dependent | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Virus |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HL257 |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S1). The exact sequence is proprietary. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2890580 |
Product Specific Information
- Positive control: 293T, SARS-CoV-2 spike (Full length)-transfected 293T, SARS-CoV-2 (2019-nCoV) Spike
- Specificity: This antibody detects SARS-CoV-2 Spike protein but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on internal testing.
Target Information
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), also known as 2019-nCoV, is the virus responsible for illnesses ranging from mild cold-like symptoms to severe respiratory distress and death.
The Spike (S) protein plays key roles in viral attachment, fusion, and entry, making it a prime target for antibody, inhibitor, and vaccine development.
The receptor-binding domain (RBD, S-RBD) of the Spike protein binds strongly to human and bat ACE2 receptors. SARS-CoV-2 RBD shows significantly higher binding affinity to ACE2 compared to SARS-CoV RBD.
The S1 subunit contains the RBD that specifically binds to ACE2 receptors on target cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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