Thermo Fisher Scientific SARS-CoV-2 Spike Protein (RBD) Recombinant Rabbit Monoclonal Antibody (HL257)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:3,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Virus

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HL257

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 Spike (S1). The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2890580

Product Specific Information

Positive control: 293T , SARS-CoV-2 spike (Full length)-transfected 293T , SARS-CoV-2 (2019-nCoV) Spike.

Specificity: This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

Target Information

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.