Thermo Fisher Scientific SOS1 Monoclonal Antibody (AF3F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

AF3F11

Immunogen

Recombinant human protein purified from E.coli (His/ABD-SOS1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538702

Product Specific Information

A suggested positive control for this product is HepG2 cells.

Target Information

Son of sevenless (SOS) is a guanine nucleotide exchange factor that activates Ras in response to growth factor stimulation. Sos1, and its closely related Sos2 paralog, are comprised of an N-terminal histone-binding domain, a RacGEF catalytic (DH) domain juxtaposed with a PH domain, a Ras exchanger motif (REM) domain, a RasGEF catalytic domain, and a C-terminal proline-rich region. Sos1 catalyzes the GDP-GTP exchange within the membrane-bound GTPase Ras and thereby switches on a key signaling circuit that involves the activation of MAPK cascade central to cellular proliferation, survival and differentiation. Growth factor stimulation rapidly induces the phosphorylation of Sos on multiple serine and threonine sites. Phosphorylation of residues within the Sos C-terminus by the MAP/ERK kinase disrupts the Grb2-Sos complex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.