Thermo Fisher Scientific Aurora B Monoclonal Antibody (AF7B11)
Aurora B 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, WB, ICC/IF, ELISA 등에 사용 가능. 2 mg/mL 농도의 액상 제품이며, A431 세포를 양성 대조군으로 권장. 연구용으로 세포 분열 및 염색체 정렬 관련 단백질 분석에 적합.
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Thermo Fisher Scientific Aurora B Monoclonal Antibody (AF7B11)
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| ELISA | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | AF7B11 |
| Immunogen | Recombinant human protein purified from E. coli (His-Aurora B) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2538694 |
Product Specific Information
A suggested positive control for this product is A431 cells.
Target Information
Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers.
This complex functions in:
- Chromosome alignment and separation
- Histone modification
- Cytokinesis
The Chromosomal Passenger Complex (CPC) includes three non-enzymatic subunits: INCENP, survivin, and borealin, which determine the activity, localization, stability, and substrate specificity of Aurora B.
Two CPCs exist during mitosis:
- One containing all four members (AurB, INCENP, survivin, borealin)
- Another consisting of INCENP and AurB
The quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for histone H3 modification.
AurB is overexpressed in several tumors, including primary breast and colon tumor samples, and its expression appears linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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