Thermo Fisher Scientific Aurora B Monoclonal Antibody (AF7B11)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

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Immunocytochemistry (ICC/IF)

5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Published species

Not Applicable

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

AF7B11

Immunogen

Recombinant human protein purified from E.coli (His-Aurora B)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538694

Product Specific Information

A suggested positive control for this product is A431 cells.

Target Information

The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.