Thermo Fisher Scientific PTPRN Monoclonal Antibody (AFT38)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

AFT38

Immunogen

Recombinant human protein purified from E.coli

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538697

Product Specific Information

A suggested positive control for this product is Myc/IA-2.

Target Information

IA-2 (insulinoma-associated protein 2, ICA512, PTPRN) is a member of the transmembrane protein tyrosine phosphatase (PTP) family located in secretory granules of neuroendocrine cells. The IA-2 protein is 979 amino acids in length and consists of a luminal domain, transmembrane domain, and cytoplasmic domain. Although a member of the PTP family, IA-2 lacks phosphatase activity with known substrates due to amino acid substitutions at critical sites in its PTP domain. Two paralog RPTPs, IA-2 and IA-2beta were identified as major autoantigens in type-1 diabetes mellitus. On granule exocytosis, the IA-2 cytoplasmic domain is cleaved and the resulting cytosolic fragment moves into the nucleus where it enhances the levels of phosphorylated STAT5 and STAT3, thereby inducing insulin gene transcription and granule biogenesis. IA-2 signaling enhances pancreatic beta-cell proliferation by regulating cyclins D through STATs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.