Thermo Fisher Scientific Cytochrome C Polyclonal Antibody
Cytochrome C 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC, Flow cytometry 등에 사용 가능. 인간, 마우스, 랫트 등 반응. 동결건조 형태로 공급되며 재구성 시 500 µg/mL 농도. 항원 친화 크로마토그래피로 정제됨.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 0.5–1 µg/mL |
| Immunohistochemistry (Frozen) (IHC-F) | 0.5–1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | 1 µg/1×10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome C (91–105 aa, ERADLIAYLKKATNE) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5 mg BSA |
| Contains | 0.05 mg sodium azide, 0.05 mg thimerosal |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746236 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Cytochrome C is an electron transport protein located in the mitochondrial intermembrane space. It plays a crucial role in oxidative phosphorylation and ATP production. Upon apoptotic stimuli, cytochrome C is released into the cytosol, where it binds apoptotic protease activating factor, leading to activation of procaspase 9 and subsequently caspase 3, a key effector of apoptosis.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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