Thermo Fisher Scientific NOX2 Polyclonal Antibody
NOX2 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 Immunohistochemistry에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, 재구성 후 500 µg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.5 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–5 µg/mL | – |
| Immunohistochemistry (Frozen) (IHC (F)) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human NOX2 (442–459aa YWLCRDTHAFEWFADLLQ) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746234 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Mox1 and the glycoprotein gp91-phox are essential components of the NADPH oxidase complex, which generates superoxide in phagocytes, neuroepithelial bodies, vascular smooth muscle cells, and endothelial cells. This complex includes membrane-bound subunits (gp91-phox and p22-phox) and cytosolic proteins (p47-phox and p67-phox).
The p22- and gp91-phox subunits also act as surface O₂ sensors initiating cellular signaling under hypoxic conditions.
Mox1 and gp91 share identical C-terminal sequence identity but differ in expression: gp91-phox is expressed in eosinophils, neutrophils, monocytes, and B-lymphocytes, whereas Mox1 is mainly found in the colon, with low expression in the uterus and prostate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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