Thermo Fisher Scientific Phospho-Ask1 (Ser83) Recombinant Rabbit Monoclonal Antibody (Ask1S83-G4)
Recombinant rabbit monoclonal antibody targeting phospho-Ask1 (Ser83). Validated for WB and Flow Cytometry. High specificity and lot-to-lot consistency with animal origin-free formulation. Suitable for human, mouse, and rat samples.
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Applications
Western Blot (WB)
- Assay-dependent
- View 1 publication
Flow Cytometry (Flow)
- 1–1,000 ng/mL
- View references
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | Ask1S83-G4 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser83 of human phospho Ask1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745027 |
Additional Formats
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
MAP3K5 (ASK1) is a serine/threonine kinase abundantly expressed in human heart and pancreas. It is activated by cytokines, growth factors, and environmental stresses. ASK1 activates MAPKKs MKK4 and MKK6, leading to activation of JNK and p38 MAP kinases. It interacts with TRAF family members and Daxx, mediating TNFR- and Fas-induced apoptosis. Overexpression induces apoptotic cell death, while inactive forms inhibit TNF-α-induced apoptosis. ASK1 is expressed in various human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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