Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, DyLight 594
염소 유래의 항-닭 IgY (H+L) 2차 항체로 DyLight 594 형광 표지. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 다중 교차흡착 처리로 비특이적 반응 최소화. 연구용으로 높은 신호 대비와 재현성 제공.
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Applications and Tested Dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Chicken IgG (IgY) – heavy and light chain |
| Conjugate | DyLight™ 594 |
| Excitation / Emission Max | 587 / 614 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556652 |
| Target | IgY |
| Cross Adsorption | Against bovine, horse, human, mouse, pig, rabbit, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, horse, human, mouse, pig, rabbit, and rat, ensuring high specificity in detection of chicken IgY targets.
Target Information
Anti-Chicken secondary antibodies are affinity-purified with well-characterized specificity for chicken immunoglobulins. They are useful for detection, sorting, or purification of chicken-derived targets.
Secondary antibodies offer increased versatility across multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity via signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 633
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- Biotin
- DyLight 350
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- TRITC
- Custom conjugation available upon request
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