Thermo Fisher Scientific Goat anti-Chicken IgY (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
염소 유래의 항-닭 IgY (H+L) 2차 항체로 DyLight 650 형광 표지. WB, IHC, ICC, Flow 등 다양한 면역실험에 사용 가능. 교차흡착 처리로 비특이적 반응 최소화. 651/673 nm 파장 특성으로 선명한 형광 신호 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Chicken IgG (IgY) – heavy and light chain |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556653 |
| Target | IgY |
| Cross Adsorption | Against bovine, horse, human, mouse, pig, rabbit, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, horse, human, mouse, pig, rabbit, and rat, ensuring high specificity in detection.
Target Information
Anti-Chicken secondary antibodies are affinity-purified antibodies with well-characterized specificity for chicken immunoglobulins.
They are useful for detection, sorting, or purification of chicken IgY.
Secondary antibodies enhance versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
Available Conjugate Formats
- Alexa Fluor 633
- Alexa Fluor Plus 405
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- Alexa Fluor Plus 594
- Alexa Fluor Plus 647
- Alexa Fluor Plus 680
- Alexa Fluor Plus 800
- AP
- Biotin
- DyLight 350
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 680
- DyLight 755
- DyLight 800
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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