Thermo Fisher Scientific Alpha-Smooth Muscle Actin Polyclonal Antibody
Western blot, IHC, ICC 등 다양한 응용에 적합한 Rabbit Polyclonal 항체. Human, Mouse, Rat 등 여러 종에 반응. 항원 친화 크로마토그래피로 정제된 고품질 제품으로 세포 구조 및 평활근 연구에 유용. 장기 보관 시 -20°C 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | - |
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 | - |
| Immunohistochemistry (Frozen) (IHC-F) | Assay-dependent | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Published Species | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.79 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7) with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792219 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)
- Positive Control: HeLa, C8D30, NIH-3T3, C2C12, Rat2, Mouse Heart
- Centrifuge briefly prior to opening the vial.
Target Information
Smooth Muscle Actin belongs to the actin family of proteins, which are highly conserved and play essential roles in cell motility, structure, and integrity.
Alpha, beta, and gamma actin isoforms have been identified:
- Alpha actin: major constituent of the contractile apparatus
- Beta and gamma actins: involved in regulation of cell motility
Smooth muscle actin (alpha actin) is found in skeletal muscle and smooth muscle vessel walls, gut wall, myometrium, and myoepithelial cells in breast and salivary glands.
Defects in the smooth muscle actin gene are associated with familial thoracic aortic aneurysm type 6.
Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells, and also appears in fibroblastic stress fibers during wound healing and fibrocontractive diseases.
Multiple alternatively spliced variants have been identified.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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