Thermo Fisher Scientific ATG4B Recombinant Rabbit Monoclonal Antibody (1HC6LC10)
ATG4B 단백질을 인식하는 재조합 토끼 단일클론 항체로, 높은 특이성과 민감도를 제공. Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 비인간 영장류 반응성. 단백질 A로 정제된 액상 형태로 4°C 또는 -20°C에 보관.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | - |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:20 | - |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 1HC6LC10 |
| Immunogen | Full-length protein corresponding to human ATG4B (UniProt ID: Q9Y4P1-1, Antigen Range: 1–393) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% Sodium Azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet Ice |
| RRID | AB_2532513 |
Product Specific Information
This antibody is predicted to react with Mouse, Bovine, and Chicken.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences are cloned from immunoreactive rabbits and screened to select optimal clones for production.
Advantages include:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal-origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene.
Autophagy is a key cellular process controlling the turnover of damaged organelles and proteins.
ATG4B acts as a deubiquitin-like protease, cleaving the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, GABARAP) to expose a C-terminal glycine.
This glycine exposure enables conjugation to phosphatidylethanolamine (PE) and membrane insertion, essential for autophagy.
ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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