Thermo Fisher Scientific OTX2 Recombinant Rabbit Monoclonal Antibody (14H14L5)
OTX2 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단클론 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며 높은 특이성과 재현성을 제공합니다. 인간, 생쥐, 랫드 등 다양한 종에 반응하며, 단백질 A로 정제된 액상 형태로 제공됩니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 14H14L5 |
| Immunogen | Peptides corresponding to Human OTX2 (aa 20–39, 276–289) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2608961 |
Product Specific Information
This antibody is predicted to react with Rat, Mouse, Pig, and Sheep.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.
Advantages include:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
OTX2 is a 289 amino acid protein belonging to the bicoid sub-family of homeodomain-containing transcription factors.
It has two known splice variants with distinct isoforms, though other isoforms may exist.
OTX2 is an early marker of endodermal differentiation of embryonic stem cells and plays a role in brain and sensory organ development.
It may bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Defects in OTX2 cause microphthalmia syndromic type 5 (MCOPS5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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