Thermo Fisher Scientific IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

-

View 2 publications 2 publications

ELISA (ELISA)

0.5-2 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP4-25D2

Conjugate

Biotin Biotin Biotin

View additional formats

• Unconjugated
• Functional Grade
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466904

Product Specific Information

Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: The MP4-25D2 antibody has been reported for use in detection of human IL-4 by ELISA and for intracellular staining for flow cytometry.

Applications Tested: The MP4-25D2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human Interleukin-4 (IL-4) in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.