Thermo Fisher Scientific IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience
인간 IL-4를 인식하는 Biotin 결합 단클론 항체로, ELISA 및 유세포분석에 적합. Th2 세포 유래 IL-4 검출에 사용. 0.5–2 µg/mL 농도 범위 권장. 4°C 보관, 동결 금지. 연구용 전용.
- 카탈로그번호
- 13-7048-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-4 Monoclonal Antibody (MP4-25D2), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | - | View 2 publications |
| ELISA | 0.5–2 µg/mL | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MP4-25D2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466904 |
Additional Formats
Product Specific Information
Description:
The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15–19 kDa cytokine secreted by Th2 cells.
Applications Reported:
Reported for use in detection of human IL-4 by ELISA and for intracellular staining in flow cytometry.
Applications Tested:
Tested as the detection antibody in a sandwich ELISA for human IL-4 analysis, in combination with the purified 8D4-8 (14-7049) antibody for capture and recombinant human IL-4 (14-8049) as the standard.
Recommended detection concentration: 0.5–2 µg/mL.
Include a standard curve with doubling dilutions of recombinant standard from 500 pg/mL to 4 pg/mL in each ELISA plate.
Filtration:
0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 has broad immunomodulatory actions and shares overlapping functions with IL-13 through receptor binding. STAT6 mediates IL-4 signaling. The IL-4 gene is located on chromosome 5q within a cytokine gene cluster including IL-3, IL-5, IL-13, and CSF2, coordinated by long-range regulatory elements. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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