Thermo Fisher Scientific IL-4 Monoclonal Antibody (BVD6-24G2), Biotin, eBioscience
마우스 IL-4 검출용 단클론 항체로 ELISA 및 유세포분석에 적합. Biotin 결합형으로 높은 특이성과 재현성을 제공. 0.5 mg/mL 농도의 액상 제품이며 4°C 암소 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific IL-4 Monoclonal Antibody (BVD6-24G2), Biotin, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry | Assay-dependent | 3 publications |
| ELISA | 0.5–2 µg/mL | 17 publications |
| Functional Assay | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BVD6-24G2 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466903 |
Additional Formats
Product Specific Information
The BVD6-24G2 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.
Applications Reported: Used for detection of mouse IL-4 by ELISA.
Applications Tested: Verified as the detection antibody in a sandwich ELISA for mouse IL-4 analysis, in combination with the purified 11B11 (14-7041) antibody for capture and recombinant mouse IL-4 (14-8041) as the standard.
Recommended concentration range for ELISA detection: 0.5–2 µg/mL.
Include a standard curve with doubling dilutions of recombinant standard (500 pg/mL–4 pg/mL) for each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 has broad immunoregulatory functions and shares overlapping activities with IL-13 through receptor binding. STAT6 mediates IL-4 signaling. The IL-4 gene is located on chromosome 5q within a cytokine cluster (IL-3, IL-5, IL-13, CSF2). IL-4, IL-13, and IL-5 are co-regulated by long-range elements over a 120 kb region. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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