Thermo Fisher Scientific IL-2 Polyclonal Antibody, Biotin, eBioscience

Applications and Tested Dilution

• ELISA (ELISA): 0.5–2 µg/mL
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Product Specifications

Product Specific Information

Description:
Rabbit anti-human IL-2 polyclonal antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported:
Reported for use in detection of human IL-2 by ELISA.

Applications Tested:
The biotinylated polyclonal rabbit anti-human IL-2 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human IL-2, in combination with the purified MQ1-17H12 (14-7029) antibody for capture and recombinant human IL-2 (14-8029) as the standard.
A suitable range of concentrations for ELISA detection is 0.5–2 µg/mL.
A standard curve with doubling dilutions of the recombinant standard over the range of 1000 pg/mL–8 pg/mL should be included in each ELISA plate.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine important for proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric complex whose gamma chain is shared by IL-4 and IL-7.
Monoallelic expression of the IL-2 gene in mature thymocytes represents a unique regulatory mechanism for precise gene control.
Disruption of an IL-2–like gene in mice leads to an ulcerative colitis–like condition, highlighting IL-2’s essential role in immune response regulation.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.