Thermo Fisher Scientific IL-2 Monoclonal Antibody (JES6-5H4), Biotin, eBioscience
Mouse IL-2를 특이적으로 인식하는 JES6-5H4 클론의 Biotin 결합 단일클론 항체입니다. ELISA, Flow cytometry, Immunoprecipitation에 사용 가능하며, 0.5 mg/mL 농도의 액상 형태로 제공됩니다. 4°C 보관, Affinity chromatography로 정제되었습니다.
- 카탈로그번호
- 13-7021-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IL-2 Monoclonal Antibody (JES6-5H4), Biotin, eBioscience
Thermo Fisher Scientific IL-2 Monoclonal Antibody (JES6-5H4), Biotin, eBioscience™
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | Assay-dependent | View 23 publications |
| ELISA | 0.5–2 µg/mL | View 19 publications |
| Immunoprecipitation (IP) | Assay-dependent | — |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | JES6-5H4 |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466898 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- APC
- APC-eFluor 780
- Brilliant UV 737
- eFluor 450
- FITC
- Functional Grade
- PE
- PE-Cyanine7
- PE-eFluor 610
- PerCP-Cyanine5.5
- Request custom conjugation
Product Specific Information
The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.
Applications Reported:
JES6-5H4 has been reported for use in detection of mouse IL-2 by ELISA, intracellular staining, flow cytometric analysis, immunoprecipitation, and for neutralizing IL-2 binding and biological activity.
Applications Tested:
This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-2 in combination with purified JES6-1A12 (14-7022) as the capture antibody and recombinant mouse IL-2 (14-8021) as the standard.
A suitable range of concentrations for ELISA detection is 0.5–2 µg/mL.
A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL–4 pg/mL should be included in each ELISA plate.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 2 (IL-2) is an immunomodulatory cytokine essential for proliferation of activated T cells, differentiation of B cells, NK cells, monocytes, and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric complex whose gamma chain is shared with IL-4 and IL-7.
Expression of the IL-2 gene in mature thymocytes is monoallelic, representing a unique regulatory mechanism for precise gene expression.
Disruption of an IL-2-like gene in mice leads to an ulcerative colitis-like disease, indicating its critical role in immune response regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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