Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 650 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 plays a key role in peripheral tolerance and autoimmune disease regulation in mice. It is mainly expressed on activated T and B lymphocytes and interacts with PD-L1 (B7-H1) and PD-L2 (B7-DC).
J43 inhibits the binding of PD-L1-Ig and PD-L2-Ig to PD-1 transfected cells and can modulate immune responses in vivo.
This antibody is validated for flow cytometric analysis of mouse splenocytes, recommended at ≤0.5 µg/test. Optimal titration is advised.

Brilliant Violet™ 605 (BV605) emits at 605 nm and is designed for cytometers equipped with a violet (405 nm) laser.
For multi-color staining, use Super Bright Complete Staining Buffer or Brilliant Stain Buffer™ to minimize non-specific polymer interactions.

• Light sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Samples can be stored up to 3 days at 4°C in IC Fixation Buffer or 1-step Fix/Lyse Solution. BV605 is not compatible with methanol-based fixation.
• Excitation: 407 nm
• Emission: 605 nm
• Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.™

Target Information

PD-1 (CD279) is an inhibitory receptor expressed on activated T cells, mediating immune downregulation upon binding to its ligands PD-L1 and PD-L2. This interaction limits T-cell activation in the absence of danger signals. PD-1 deficiency leads to hyperactivation and autoimmune manifestations in mice. PD-1 often migrates at a higher molecular weight in SDS-PAGE despite its predicted size.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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