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Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience
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Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience

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인간 IgM의 μ 중쇄를 인식하는 SA-DA4 단클론 항체로, Brilliant Violet 605 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 비특이적 염색을 최소화하기 위해 sodium citrate 사용을 권장합니다. 4°C 암소 보관, 동결 금지.

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406-9998-4x (2개 옵션)
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Thermo Fisher Scientific 406-9998-42 IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience 100 tests pk판매 단위 pk ·
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Thermo Fisher Scientific 406-9998-41 IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience 25 tests pk판매 단위 pk ·
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Thermo Fisher Scientific · Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience

Applications

  • Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.25 µg)/test
Publications: -


Product Specifications

항목 내용
Species Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Recommended Isotype Control Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 605, eBioscience™
Class Monoclonal
Type Antibody
Clone SA-DA4
Conjugate Brilliant Violet™ 605
Excitation / Emission Max 406 / 603 nm
Form Liquid
Concentration 5 µL/Test
Purification Affinity chromatography
Storage Buffer PBS, pH 7.2, with BSA
Contains 0.09% sodium azide
Storage Conditions 4°C, store in dark, DO NOT FREEZE
Shipping Conditions Wet ice
RRID AB_3093235

Additional Formats

  • Unconjugated
  • APC
  • Biotin
  • Brilliant UV 395
  • Brilliant UV 737
  • Brilliant Violet 480
  • Brilliant Violet 786
  • eFluor 450
  • FITC
  • PE
  • PE-Cyanine7
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Request custom conjugation

Product Specific Information

The SA-DA4 monoclonal antibody reacts with the μ heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells.

Testing has revealed variations in staining of non-B cell populations, likely due to donor variability and anticoagulant choice. Sodium citrate is recommended to minimize non-specific staining.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test (final volume 100 µL).

Brilliant Violet™ 605 (BV605) emits at 605 nm and is designed for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.

When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
BV605 is not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.


Target Information

IgM is expressed intracellularly during early B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral blood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.


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