
Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience
인간 IgM의 μ 중쇄를 인식하는 SA-DA4 단클론 항체로, Brilliant Violet 605 형광체가 결합되어 있습니다. Flow Cytometry에 최적화되어 있으며, 비특이적 염색을 최소화하기 위해 sodium citrate 사용을 권장합니다. 4°C 암소 보관, 동결 금지.
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Thermo Fisher Scientific IgM Monoclonal Antibody (SA-DA4), Brilliant Violet 605, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Violet™ 605, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SA-DA4 |
| Conjugate | Brilliant Violet™ 605 |
| Excitation / Emission Max | 406 / 603 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_3093235 |
Additional Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 395
- Brilliant UV 737
- Brilliant Violet 480
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The SA-DA4 monoclonal antibody reacts with the μ heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly during early stages of B lymphopoiesis and on the surface of mature B cells.
Testing has revealed variations in staining of non-B cell populations, likely due to donor variability and anticoagulant choice. Sodium citrate is recommended to minimize non-specific staining.
Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-diluted and tested on normal human peripheral blood cells at 5 µL (0.25 µg) per test (final volume 100 µL).
Brilliant Violet™ 605 (BV605) emits at 605 nm and is designed for cytometers equipped with a violet (405 nm) laser. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions.
Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency.
BV605 is not compatible with methanol-based fixation.
Excitation: 407 nm
Emission: 605 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
IgM is expressed intracellularly during early B lymphopoiesis and on the surface of mature B cells in bone marrow and peripheral blood.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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