Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), Brilliant Violet 605, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532, Alexa Fluor 700
• APC, Biotin
• Brilliant UV 395, 615, 737, 805
• Brilliant Violet 421, 480, 786
• eFluor 450, FITC
• NovaFluor Blue, Red, UV, Violet, Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702
• Custom conjugation available

Product Specific Information

The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5, also known as BLR1).
CD185 is a seven-transmembrane G protein-coupled receptor expressed on B cells, CD4+ T cells, and memory T cells. It plays a crucial role in lymphocyte trafficking, particularly T cell migration into B cell follicles in response to CXCL13, making it a marker of follicular helper T cells.

• Cross-reactivity: Rhesus macaque
• Reported Applications: Flow cytometry
• Recommended Usage: 5 µL (0.5 µg) per 100 µL cell sample (10⁵–10⁸ cells/test)
• Fluorochrome: Brilliant Violet™ 605 (BV605), emits at 605 nm (Violet laser, 405 nm excitation)
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (Cat. SB-4401-42) or Brilliant Stain Buffer™ (Cat. 00-4409-75) to minimize non-specific interactions.
• Light Sensitivity: Protect from light.
• Fixation Compatibility: Compatible with IC Fixation Buffer (Cat. 00-8222-49) or 1-step Fix/Lyse Solution (Cat. 00-5333-54). Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 605 nm
Laser: Violet laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Target: CXCR5 (C-X-C Chemokine Receptor Type 5, CD185, BLR1)
CXCR5 is a G protein-coupled receptor for chemokine BLC, expressed in Burkitt’s lymphoma and lymphoid tissues such as lymph nodes and spleen. It regulates B cell migration and is present in various tissues including blood, bone marrow, brain, liver, and tonsil. CXCR5 belongs to the GPCR family, which mediates signal transduction via G-proteins. GPCRs are major pharmacological targets, with several drugs developed based on this mechanism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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