Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2463)
인간 Ki-67 단백질을 인식하는 Mouse monoclonal antibody로, 세포 증식 마커 연구에 적합. Western blot, IHC, Flow cytometry 등 다양한 응용 가능. 고순도 Protein A/G 정제, 4°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific Ki-67 Monoclonal Antibody (MKI67, 2463)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MKI67, 2463 |
| Immunogen | Recombinant fragment of human Ki67 protein (around aa 2293–2478) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Ki-67 is a nuclear protein expressed during the late G1, S, G2, and M phases of the cell cycle. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Expression pattern:
- G1: Perinucleolar expression
- G2: Nuclear matrix expression
- M phase: Chromosomal expression
Ki-67 serves as a reliable proliferation marker since it is absent in G0 cells and increases progressively through mitosis. It is used to determine the growth fraction in neoplasms and has strong prognostic correlation with tumor grade. Commonly utilized as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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