Thermo Fisher Scientific Ki-67 (Proliferating Cell Marker) Recombinant Mouse Monoclonal Antibody (rMKI67/4948)
Ki-67 단백질을 표적하는 재조합 마우스 단클론 항체로, 세포 증식 마커 연구에 적합합니다. Western blot 및 ELISA에 사용 가능하며, 인간 시료에 반응합니다. 핵 내 발현 단백질 검출에 유용하며, 단백질 A로 정제된 액상 항체입니다.
- 카탈로그번호
- 4288-MSM35-P1ABX
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| ELISA | 2–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Expression System | CHO cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMKI67/4948 |
| Immunogen | Recombinant fragment (around aa2200–2500) of human MKI67 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Dry ice (international) |
Product Specific Information
- Positive Control: Any actively proliferating cells such as human skin, tonsil, or lymph node
- Cellular Location: Nucleus
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. The protein contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and the nucleolus.
Ki-67 shows cell cycle-dependent distribution:
- Perinucleolar expression at G1
- Nuclear matrix localization at G2
- Chromosomal association during M phase
Because Ki-67 is absent in G0 cells and increases from G1 through mitosis, it serves as a reliable proliferation marker. Ki-67 antibodies are useful for determining the growth fraction of neoplastic cells, showing strong correlation between low Ki-67 index and low-grade tumors. It is routinely used as a neuronal marker for cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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