Thermo Fisher Scientific SCO2 Polyclonal Antibody
Human SCO2 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot과 IHC에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며 PBS 완충액에 0.02% sodium azide 포함. 인간 간 조직 lysate를 양성 대조군으로 권장. 연구용으로만 사용.
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Thermo Fisher Scientific SCO2 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–2 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 19 amino acid peptide from near the carboxy terminus of human SCO2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at −20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153513 |
Product Specific Information
- Suggested positive control: human liver tissue lysate
- PA5-20339 can be used with blocking peptide PEP-0459
Target Information
Synthesis of cytochrome c oxidase 2 (SCO2) was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane.
Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding.
Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression—such as in numerous tumors and cell lines—leads to reduced SCO2 expression and a shift from normal aerobic respiration towards glycolytic ATP production.
Defects in SCO2 are associated with fatal infantile cardioencephalomyopathy and COX deficiency.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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