Thermo Fisher Scientific DRAM Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2-2.5 µg/mL

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 16 amino acid peptide from near the amino terminus of human DRAM. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DRAM,uniProtId:Q8N682-1,ncbiNodeId:9606,antigenRange:1,antigenLength:238,antigenImageFileName:PA5-20336_DRAM_Q8N682-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20336_DRAM_Q8N682-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153555

Product Specific Information

A suggested positive control is K562 cell lysate.

PA5-20336 can be used with blocking peptide PEP-0456.

Target Information

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.