Thermo Fisher Scientific CUG-BP2 Monoclonal Antibody (9B1)
CUG-BP2 단백질 검출용 Mouse Monoclonal Antibody (Clone 9B1). Western blot, IHC, Flow cytometry에 적합. Human, Mouse, Rat 반응성. 고순도 Protein G 정제, 장기 보관 시 -20°C 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9B1 |
| Immunogen | Synthetic peptide within Human CELF2 aa 460–508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2911552 |
Product Specific Information
- Positive Control: PC-3M cell lysates, rat skeletal muscle tissue, mouse colon tissue, Daudi
- Subcellular Location: Cytoplasm, Nucleus
Target Information
CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)₈ oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50.
CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor.
CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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