Thermo Fisher Scientific CXCL14 Polyclonal Antibody, Biotin, PeproTech

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.2 µg/mL

-

ELISA (ELISA)

0.25-1.0 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human BRAK (CXCL14) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CXCL14,uniProtId:O95715-1,ncbiNodeId:9606,antigenRange:35-111,antigenLength:111,antigenImageFileName:500-P237BT-25UG_CXCL14_O95715-1_Rabbit.svg,antigenImageFileNamePDP:500-P237BT-25UG_CXCL14_O95715-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2929976

Product Specific Information

AA Sequence of recombinant protein: SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hBRAK by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BRAK (500-P237) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBRAK.

Western Blot: To detect hBRAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBRAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P237BT-1MG will be provided as 2 x 500 µg

Target Information

This gene belongs to the cytokine gene family which encode secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils or macrophages. It has been implicated that this cytokine is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.