
Thermo Fisher Scientific CXCL14 Polyclonal Antibody, PeproTech
CXCL14 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체로, Western blot, ELISA, IHC 등 다양한 분석에 사용 가능. 항원 친화 크로마토그래피로 정제된 고순도 항체이며, 인간 및 마우스 반응성이 확인됨. 냉동(-20°C) 보관.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | - |
| Immunohistochemistry (IHC) | - | 2 publications |
| ELISA | 0.5–2.0 µg/mL | - |
| Immunostaining (IS) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Mouse |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human BRAK (CXCL14) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2929972 |
Product Specific Information
AA Sequence of recombinant protein:
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human BRAK (CXCL14) matrix.
Sandwich ELISA:
To detect Human BRAK (CXCL14) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BRAK (CXCL14) (500-P237Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BRAK (CXCL14).
Western Blot:
To detect Human BRAK (CXCL14) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human BRAK (CXCL14) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
This gene belongs to the cytokine gene family which encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines, characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils, or macrophages. It is implicated in the homeostasis of monocyte-derived macrophages rather than inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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