Thermo Fisher Scientific CXCL14 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human BRAK (CXCL14) matrix.

Sandwich ELISA:
To detect Human BRAK (CXCL14) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BRAK (CXCL14) (500-P237Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BRAK (CXCL14).

Western Blot:
To detect Human BRAK (CXCL14) by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human BRAK (CXCL14) is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

This gene belongs to the cytokine gene family which encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines, characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils, or macrophages. It is implicated in the homeostasis of monocyte-derived macrophages rather than inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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