Thermo Fisher Scientific CXCL14 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIITTKS VSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BRAK (CXCL14). Anti-Human BRAK (CXCL14)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hBRAK by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BRAK (500-P237) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hBRAK.

Western Blot:
To detect hBRAK by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBRAK is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P237BT-1MG will be provided as 2 × 500 µg.

Target Information

This gene belongs to the cytokine gene family, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC (Cys-X-Cys) subfamily of cytokines, characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes but not for lymphocytes, dendritic cells, neutrophils, or macrophages. It is implicated in the homeostasis of monocyte-derived macrophages rather than in inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.