Thermo Fisher Scientific SMAD2 Monoclonal Antibody (3G9)
SMAD2 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 3G9). Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 적합. 인간 SMAD2에 반응하며, Affinity chromatography로 정제된 액상 형태. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G9 |
| Immunogen | SMAD2 (AAH25699, 181–280 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
PRHTEILTEL PPLDDYTHSI PENTNFPAGI EPQSNYIPET PPPGYISEDG ETSDQQLNQS MDTGSPAELS PTTLSPVNHS LDLQPVTYSE PAFWCSIAYY
Target Information
SMAD2 (MADH2, MAD2) regulates multiple cellular processes, including cell proliferation, apoptosis, and differentiation. It interacts with TGF-beta receptors through the SMAD anchor for receptor activation, enabling nuclear translocation—a central event in TGF-beta signaling. Phosphorylation of threonine 8 in the MH1 domain by ERK1 enhances transcriptional activity, negatively regulated by calmodulin. Upon TGF-beta stimulation, SMAD2 is phosphorylated, dissociates from SARA, and associates with SMAD4 for nuclear translocation and transcriptional regulation. SMAD2 can also mediate activin signaling via activin type 1 receptor kinase. Alternatively spliced transcript variants encoding SMAD2 have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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