Thermo Fisher Scientific PIWIL2 Monoclonal Antibody (11C11)
PIWIL2 단백질 검출용 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 11C11). Western blot, ELISA, Immunoprecipitation에 사용 가능. 1 mg/mL 농도의 액상 형태로, -20°C에서 보관. 생식세포 및 줄기세포 관련 연구에 적합.
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Thermo Fisher Scientific PIWIL2 Monoclonal Antibody (11C11)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 5 µg |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11C11 |
| Immunogen | Recombinant human protein purified from E.coli (GST-mili-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2538676 |
Product Specific Information
A suggested positive control for this product is mouse testis.
Target Information
The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family is required for germ and stem cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like, Piwil2, Piwi-like protein 2), are essential for spermatogenesis in mouse.
MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26–31 nucleotides in length, distinct from the 21–23 nucleotides of microRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila.
MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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