Thermo Fisher Scientific PIWIL2 Monoclonal Antibody (11C11)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

ELISA (ELISA)

Assay-dependent

-

Immunoprecipitation (IP)

5 µg

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

11C11

Immunogen

Recombinant human protein purified from E.coli (GST-mili-1)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2538676

Product Specific Information

A suggested positive control for this product is mouse testis.

Target Information

The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family is required for germ and stem cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like, Piwil2, Piwi-like protein 2), are essential for spermatogenesis in mouse. MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26-31 nucleotides in length clearly distinct from the 21-23 nucleotides of microRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.