Thermo Fisher Scientific RSK2/RSK3 Monoclonal Antibody (3C1)

Applications

Western Blot (WB)

• Tested Dilution: 2 µg/mL

ELISA

• Tested Dilution: Assay-dependent

Product Specifications

Product Specific Information

A suggested positive control for this product is HeLa cells.

Target Information

The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1–4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. These kinase domains are activated sequentially by a series of phosphorylations.

RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFκB/IkBα, and CREB. ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in both the cytoplasm and nucleus. Growth factor stimulation results in activation of both cytosolic and nuclear RSK and translocation of the cytosolic RSK into the nucleus upon activation. This activation and nuclear translocation lead to phosphorylation and activation of transcription factors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.