Thermo Fisher Scientific WHSC2 Monoclonal Antibody (6B11H8)
WHSC2 단백질을 인식하는 Mouse monoclonal antibody (clone 6B11H8). Human 및 Rat 시료에서 WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능. Protein G 정제, PBS 버퍼, 1 mg/mL 농도. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | – |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | – |
| Flow Cytometry (Flow) | 1:200–1:400 | – |
| ELISA | 1:10,000 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6B11H8 |
| Immunogen | Purified recombinant fragment of human WHSC2 (amino acids 280–511) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538670 |
Product Specific Information
MA5-17199 targets WHSC2 in indirect ELISA, FACS, ICC, IHC, IF, and WB applications and shows reactivity with Human and Rat samples.
The immunogen is a purified recombinant fragment of human WHSC2 (amino acids 280–511) expressed in E. coli.
MA5-17199 detects WHSC2 with a predicted molecular weight of approximately 57.3 kDa.
Target Information
The WHSC2 gene is ubiquitously expressed, with higher levels in fetal than adult tissues. It encodes a protein sharing 93% sequence identity with the mouse homolog.
Wolf-Hirschhorn syndrome (WHS) is associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene maps to the 165 kb WHS critical region and may contribute to the WHS or Pitt-Rogers-Danks syndrome phenotype.
The encoded protein reacts with HLA-A2-restricted, tumor-specific cytotoxic T lymphocytes, suggesting potential use in cancer immunotherapy.
It is also a component of the NELF (negative elongation factor) complex involved in RNA polymerase II transcription regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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