Thermo Fisher Scientific FLT3LG Polyclonal Antibody, PeproTech
FLT3LG 단백질을 인식하는 토끼 폴리클로날 항체로, 인간 시료에 반응하며 WB 및 ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 민감도를 제공. 연구용으로 세포 신호 및 면역 반응 분석에 적합.
- 카탈로그번호
- 500-P42-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific FLT3LG Polyclonal Antibody, PeproTech
Thermo Fisher Scientific FLT3LG Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.5–2.0 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Flt3-Ligand |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930533 |
Product Specific Information
AA Sequence of recombinant protein:
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Flt3-Ligand. Anti-Human Flt3-Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human Flt3-Ligand matrix.
Sandwich ELISA:
To detect Human Flt3-Ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Flt3-Ligand (500-P42Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Flt3-Ligand.
Western Blot:
To detect Human Flt3-Ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Flt3-Ligand is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a protein-coding gene. Diseases associated with FLT3LG include aplastic anemia and brain cancer. Related pathways include hematopoietic stem cell differentiation and Ras signaling. Gene Ontology (GO) annotations include protein homodimerization activity and cytokine activity.
Dendritic cells (DCs) link innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs, particularly plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.
Note: For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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