Thermo Fisher Scientific M-CSF Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF. Anti-Human M-CSF-specific antibody was purified by affinity chromatography employing an immobilized Human M-CSF matrix.

Sandwich ELISA:
To detect Human M-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity-purified antibody, in conjunction with PeproTech Biotinylated Anti-Human M-CSF (500-P44Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human M-CSF.

Western Blot:
To detect Human M-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human M-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Target Information

M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
It induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization.
M-CSF exists as several bioactive isoforms differing in potency and stability. The full-length protein is synthesized as a membrane-spanning form, which can be cleaved and secreted.
It functions as a disulfide-bonded homodimer and can exist as a glycoprotein or proteoglycan modified with chondroitin sulfate.
Binding to its receptor c-Fms (CSF-1R/CD115) leads to receptor dimerization, internalization, and degradation.
Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte–macrophage lineages.
Human M-CSF shows activity on mouse cells, but mouse CSF has no activity on human cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.