Thermo Fisher Scientific M-CSF Polyclonal Antibody, PeproTech
Human M-CSF를 인식하는 Rabbit Polyclonal Antibody로, Western Blot과 ELISA에 최적화되어 있습니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 연구용으로 Human M-CSF의 정량 및 검출에 적합합니다.
- 카탈로그번호
- 500-P44-xxx (3개 옵션)
- 판매단위
- pk
카탈로그
3개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific M-CSF Polyclonal Antibody, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | View 1 publication |
| ELISA | 0.5–2.0 µg/mL | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human M-CSF |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930545 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human M-CSF. Anti-Human M-CSF-specific antibody was purified by affinity chromatography employing an immobilized Human M-CSF matrix.
Sandwich ELISA:
To detect Human M-CSF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity-purified antibody, in conjunction with PeproTech Biotinylated Anti-Human M-CSF (500-P44Bt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human M-CSF.
Western Blot:
To detect Human M-CSF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Human M-CSF is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Target Information
M-CSF (Macrophage Colony-Stimulating Factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
It induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization.
M-CSF exists as several bioactive isoforms differing in potency and stability. The full-length protein is synthesized as a membrane-spanning form, which can be cleaved and secreted.
It functions as a disulfide-bonded homodimer and can exist as a glycoprotein or proteoglycan modified with chondroitin sulfate.
Binding to its receptor c-Fms (CSF-1R/CD115) leads to receptor dimerization, internalization, and degradation.
Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte–macrophage lineages.
Human M-CSF shows activity on mouse cells, but mouse CSF has no activity on human cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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