Thermo Fisher Scientific FLT3LG Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific FLT3LG Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Flt3-ligand. Anti-Human Flt3-ligand-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human Flt3-ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Flt3-ligand (500-P42) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Flt3-ligand.

Western Blot:
To detect Human Flt3-ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant Human Flt3-ligand is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P42BT-1MG will be provided as 2 × 500 µg.

Target Information

FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a protein-coding gene.
Diseases associated with FLT3LG include Aplastic Anemia and Brain Cancer.
Related pathways include Hematopoietic Stem Cell Differentiation and Ras signaling pathway.
Gene Ontology (GO) annotations: protein homodimerization activity and cytokine activity.
Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses.
FLT3LG controls the development of DCs, particularly plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.