Thermo Fisher Scientific FLT3LG Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 FLT3LG 폴리클로날 항체로 인간 Flt3-ligand 검출에 최적화. Western blot 및 Sandwich ELISA에 사용 가능. 고순도 항원 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 연구용으로만 사용.
- 카탈로그번호
- 500-P42BT-xxxx (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific FLT3LG Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human Flt3-Ligand |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930537 |
Product Specific Information
AA Sequence of recombinant protein:
TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human Flt3-ligand. Anti-Human Flt3-ligand-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human Flt3-ligand by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Flt3-ligand (500-P42) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human Flt3-ligand.
Western Blot:
To detect Human Flt3-ligand by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human Flt3-ligand is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Package Information:
500-P42BT-1MG will be provided as 2 × 500 µg.
Target Information
FLT3LG (Fms Related Tyrosine Kinase 3 Ligand) is a protein-coding gene. Diseases associated with FLT3LG include Aplastic Anemia and Brain Cancer. Related pathways include Hematopoietic Stem Cell Differentiation Pathways, Lineage-specific Markers, and Ras signaling pathway.
Gene Ontology (GO) annotations: protein homodimerization activity, cytokine activity.
Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3LG controls the development of DCs and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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